Journal: Cureus
Article Title: Dexmedetomidine Inhibits Hippocampal Neuronal Damage Caused by Persistent Isoflurane-Induced Hypotension in Rat Model of Chronic Cerebral Hypoperfusion
doi: 10.7759/cureus.61522
Figure Lengend Snippet: (a) Photomicrograph of both NeuN and MAP2 in the hippocampal CA1 region in the NN (normotension + no dexmedetomidine) group, ND (normotension + dexmedetomidine) group, HN (hypotension + no dexmedetomidine) group, and HD (hypotension + dexmedetomidine) group. (b) Box and whisker plots depicting the counts of normal neuronal cells in predefined areas (0.04 mm2) within the hippocampal CA1 region across the NN, ND, HN, and HD groups. The values are presented as the median (represented by the transverse line inside the boxes), interquartile range (IQR) denoted by the boxes, and the 10th–90th percentiles illustrated by the whiskers. NN and ND groups did not show a reduction in normal cell counts, but they were significantly decreased in the HN group. The HN group showed a significant reduction in the MAP2-positive area of the hippocampal CA1 region, but dexmedetomidine pretreatment protected against the reduction. *P < 0.05 vs. NN and HD groups
Article Snippet: The sections were then washed three times with TBS-T, and incubated overnight at room temperature with mouse monoclonal antibodies against neuronal nuclei (NeuN) (Millipore, Billerica, MA, USA), as a neuronal marker that recognizes nuclear proteins, microtubule-associated protein-2 (MAP-2) (Sigma Aldrich, Burlington, MA, USA), which is located almost exclusively in the neuronal perikaryal and dendrites and the reduction of which indicates neuronal damage, and ionized calcium-binding adaptor molecule 1 (Iba1) (Wako Pure Chemical, Osaka, Japan), as a marker of microglia, or rabbit polyclonal antibody against glial fibrillary acidic protein (GFAP) (DAKO, Glostrup, Denmark), as a marker of astrocytes.
Techniques: Whisker Assay
